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Topical Workshop on Lignocellulolytic Enzymes: Production and Application

19/03/2018 - 12:19


         Topical Workshop on Lignocellulolytic Enzymes: Production and Application was held by the Food Industries Research Institute in collaboration with Chalmers University of Technology in Hoa Binh, Vietnam from 6th to 7th February, 2018.

         Prof. Dr. Le Duc Manh, the Director of FIRI delivered welcome speech to delegates and participants. He said that lignocellulose was the most abundant organic material on Earth. It represents an enormous and sustainable source of energy and raw materials for processing industries. One of the major difficulties is the resistance of lignocellulose to the actions of modifying enzymes. Vietnam is located in the tropical region with highest microbial diversity and fastest carbon cycle. It is expected that the tropics can provide a number of interesting and unstudied lignocellulose modifying enzymes. The workshop provides an opportunity for researchers interested in the field to present, exchange ideas and visions on the production and application of lignocellulolytic enzymes.


Prof. Dr. Le Duc Manh, the Director of FIRI delivered welcome speech to participants


       The keynote speech Prof. Lisbeth Olsson from Chalmers University of Technology gave presentation on “Enzymes in the service of bioeconomic solutions- hydrolysis, modification and upgrading of lignocellulose”. Chalmers University of Technology has been cooperating with the Food Industries Research Institute in two projects since 2010, i.e. “Engineering of new lignocellulolytic enzymes for feed and bio-ethanol production from agricultural waste” & “Enzyme discovery and production in Vietnam for sustainable production of biochemicals and biofuels”. The cooperation includes isolation of fungal strains, new enzyme discovery, genomics and training etc. These projects focused on (a) enzymes that could degrade plant cell wall materials and understanding enzymatic hydrolysis, chemical and structural changes during the process; (b) enzymes that could modify plant cell wall material for production of materials with modified surface; (c) enzymes that could upgrade plant cell wall material valuable chemicals.


Discussion during the Workshop


      During 8 years of collaboration between Chalmers University of Technology and the Food Industries Research Institute, 1100 strains of mesophilic and 106 strains  of thermophilic fungi were isolated. The strains were identified based on rRNA gene sequencing and 29 species were detected. The presence of 23 undescribed genetic groups of thermophilic fungi was revealed. Fungal secretomes were studied for the presence of accessory enzymes and the capability of degrading untreated rice straw. A number of strains showed higher hydrolytic activity than the well-known Trichoderma reesei RUT C30 strain. Genome and transcriptome of strains growing on different substrates were investigated in order to identify enzyme systems involved in lignocellulose degradation. An example of recombinant enzyme production for application in feed industry was presented.

        Experts from universities and research institutions in Hanoi areas and Sweden also gave interesting presentations in this field.


Participants join in the Topical Workshop on Lignocellulolytic Enzymes: Production and Application

                                                                                                               (Photo by Dinh Duc Hien)


             After two working days, the Topical Workshop on Lignocellulolytic Enzymes: Production and Application was held successfully and provided interesting information in this field to experts and participants.




                                                                                                                                              By Khuat Thi Thuy





The workshop program

February 6, 2018

16:00-16:10: Welcome speech by Director of FIRI, Assoc. prof. Le Duc Manh;

16:10-16:50: Introductory seminar (Prof. Lisbeth Olson, Chalmers);

16:50- 17:20: Presentation on “Bio refinery: possible exploration of lignocellulose waste in Vietnam” (Dr. Tran Tuan Anh, Hanoi University of Science and Technology);

17:30-18:00: Presentation on “Genome transcriptome analysis of the thermophilic fungus Malachi Cinnamon (Dr. Silvia Hotter, Chalmers University of Technology);

18:00-18h:30: Presentation on “Investigation of enzyme chitinase screened from VN type culture collection and their potential application in producing pesticide” (Dr. Tran Thi Van Anh, Vietnam Type Culture Collection)


February 7, 2018

8:30-9h:00: Presentation on “Development of new and highly efficient Agrobacterium tumefaciens-mediated transformation system for genetic engineering of filamentous fungi” (Dr. Tran Van Tuan, Vietnam National University);

9:00-9:30: Presentation on “Biochemical and Structural diversity in Carbohydrate Esterase family 15 and  its implications for cleavage of lignin-carbohydrates” (Assoc. Prof. Johan Larsbrink, Chalmers University of Technology);

9:40-10:10: Presentation on “Lignocellulolytic enzyme studies at Microbiology and Biotechnology Department” (Dr. Duong Minh Lam, from Hanoi University of Education);

10:10-10:40: Presentation on “Exploring fungal diversity of Vietnam for novel lignocellulolytic enzymes” (Assoc. prof. Vu Nguyen Thanh, the Food Industries Research Institute);


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